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  • 1.  Cell based assay

    Posted 07-17-2025 11:47
    Hello group

    Our hospital sends NMO and MOG testing
    to Quest laboratories
    Quest laboratories performs a cell based flow cytometry
    It is a fluorescence activated cell- sorting Assay 

    They are currently refusing to send this testing to the Mayo Clinic.

    IS there any written evidence to say that sending NMO and MOG testing to the Mayo Clinic is more sensitive and specific because of their particular cell based Assay?

    Thank you
    Michael Altman 
    Baltimore 

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  • 2.  RE: Cell based assay

    Posted 07-17-2025 11:57

    Michael,

    I think Quest does a fixed cell-based assay as opposed to a live cell-based assay.  It is quite good (much better than ELISA, etc) and should be the starting point if that is your hospital's default.  However, if you get results that do not seem to meet the clinical picture, I think you could argue to have it sent to a lab that does a live cell-based assay, such as Mayo.

    You could cite these papers:

    A multicenter comparison of MOG-IgG cell-based assays | Neurology

    Serologic diagnosis of NMO | Neurology

    Real‐world clinical experience with serum MOG and AQP4 antibody testing by live versus fixed cell‐based assay - Said - 2025 - Annals of Clinical and Translational Neurology - Wiley Online Library

    Best,

    John

     




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  • 3.  RE: Cell based assay

    Posted 07-17-2025 14:26

    Quest does fixed- and live-cell assays for MOG-IgG

    The live cell assay is only available in certain regions (check web).

    Fixed-cell assay is less-sensitive but a reasonable starting point as John stated.

     

    Jeff

     

    Jeffrey Bennett, M.D., Ph.D.
    Gertrude Gilden Professor 
    Vice Chair, Basic Research - Neurology
    Departments of Neurology & Ophthalmology
    Programs in Neuroscience & Immunology
    University of Colorado School of Medicine
    Research Complex 2, Room 5001

    Voice: 303-724-2184
    Fax: 303-724-2213




    Original Message


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